Publikationen

2021

Belyaev I, Praetorius JP, Medyukhina A, Figge MT (2021) Enhanced segmentation of label-free cells for automated migration and interaction tracking. Cytometry A, 1-12.

Capocefalo A, Deckert-Gaudig T, Brasili F, Postorino P, Deckert, V (2021) Unveiling the interaction of protein fibrils with gold nanoparticles by plasmon enhanced nano-spectroscopy. Nanoscale 13, 14469–14479.

Cialla-May D, Gadkari J, Winterfeld A, Hübner U, Weber K, Diekert G, Schubert T, Goris T, Popp J (2021) Surface enhanced Raman spectroscopy-based evaluation of the membrane protein composition of the organohalide-respiring Sulfurospirillum multivorans. J Raman Spectrosc 52(2), 458–467.

Darussalam E, Peterfi O, Deckert-Gaudig T, Roussille L, Deckert, V (2021) pH-dependent disintegration of insulin amyloid fibrils monitored with atomic force microscopy and surface-enhanced Raman spectroscopy. Spectrochim Acta A 256, 119672.

D'Enfert C, Kaune AK, Alaban LR, Chakraborty S, Cole N, Delavy M, Kosmala D, Marsaux B, Fróis-Martins R, Morelli M, Rosati D, Valentine M, Xie Z, Emritloll Y, Warn PA, Bequet F, Bougnoux ME, Bornes S, Gresnigt MS, Hube B, Jacobsen ID, Legrand M, Leibundgut-Landmann S, Manichanh C, Munro CA, Netea MG, Queiroz K, Roget K, Thomas V, Thoral C, Van den Abbeele P, Walker AW, Brown AJP (2021) The impact of the Fungus-Host-Microbiota interplay upon Candida albicans infections: current knowledge and new perspectives. FEMS Microbiol Rev 45(3), fuaa060.

Ebert C, Tuchscherr L, Unger N, Pöllath C, Gladigau F, Popp J, Löffler B, Neugebauer U (2021) Correlation of crystal violet biofilm test results of Staphylococcus aureus clinical isolates with Raman spectroscopic read-out. J Raman Spectrosc, doi.org/10.1002/jrs.6237.

Gottschall T, Meyer-Zedler T, Schmitt M, Huber R, Popp J, Tünnermann A, Limpert J (2021) Ultra-compact tunable fiber laser for coherent anti-Stokes Raman imaging. J Raman Spectrosc 52, 1561-1568.

Hassan MIA, Keller M, Hillger M, Binder U, Reuter S, Herold K, Telagathotia A, Dahse HM, Wicht S, Trinks N, Nietzsche S, Deckert-Gaudigh T, Deckert V, Mrowka R, Kerpitz U, Saluz HP, Voigt K (2021) The impact of episporic modification of Lichtheimia corymbifera on virulence and interaction with phagocytes. Comput Struct Biotechnol J 19, 880–896.

Kästner B, Hengoju S, Svensson CM, Figge MT, Rosenbaum MA (2021) Mit Tropfenmikrofluidik zu Hochgeschwindigkeits-Biotechnologie. BIOspektrum 27(3), 260-262.

König A, Müller R, Mogavero S, Hube B (2021) Fungal factors involved in host immune evasion, modulation and exploitation during infection. Cell Microbiol 23(1), e13272.

Last A, Maurer M, Mosig AS, Gresnigt MS, Hube B (2021) In vitro infection models to study fungal–host interactions. FEMS Microbiol Rev (45)5, fuab005.

Machata S, Sreekantapuram S, Hünniger K, Kurzai O, Dunker C, Schubert K, Krüger W, Schulze-Richter B, Speth C, Rambach G, Jacobsen ID (2021) Significant differences in host-pathogen interactions between murine and human whole blood. Front Immunol 11, 565869.

Mogavero S, Sauer FM, Brunke S, Allert S, Schulz D, Wisgott S, Jablonowski N, Elshafee O, Krüger T, Kniemeyer O, Brakhage AA, Naglik JR, Dolk E, Hube B (2021) Candidalysin delivery to the invasion pocket is critical for host epithelial damage induced by Candida albicans. Cell Microbiol 23(10), e13378.

Plidschun M, Ren H, Kim J, Förster R, Maier SA, Schmidt MA (2021) Ultrahigh numerical aperture meta-fibre for flexible optical trapping. Light Sci Appl (10)1, 57.

Pistiki A, Ramoji A, Ryabchykov O, Thomas-Rüddel D, Press AT, Makarewicz O, Giamarellos-Bourboulis EJ, Bauer M, Bocklitz T, Popp J, Neugebauer U (2021) Biochemical analysis of leukocytes after in vitro and in vivo activation with bacterial and fungal pathogens using Raman spectroscopy. Int J Mol Sci 22(19), 10481.

Ramoji A, Thomas-Rüddel D, Ryabchykov O, Bauer M, Arend N, Giamarellos-Bourboulis EJ, Eugen-Olsen J, Kiehntopf M, Bocklitz T, Popp J, Bloos F, Neugebauer U (2021) Leukocyte activation profile assessed by Raman spectroscopy helps diagnosing infection and sepsis. Crit Care Explor 3(5), e0394.

Rujas E, SaraInsausti DPL, Carravilla P, García-Porras M, Largo E, Morillo I, Sánchez-Eugenia R, Zhang L, Cui H, Iloro I, Elortza F, Julien JP, Eggeling C, Zwick MB, Caaveiro JMM, Nieva JL (2021) Focal accumulation of aromaticity at the CDRH3 loop mitigates 4E10 polyreactivity without altering its HIV neutralization profile. iScience (24)9, 102987.

Sreekantapuram S, Berens C, Barth SA, Methner U, Berndt A (2021) Interaction of Salmonella Gallinarum and Salmonella Enteritidis with peripheral leucocytes of hens with different laying performance. Vet Res 52(1), 123.

Tannert A, Lopez JG, Petkov N, Ivanova A, Peneva K, Neugebauer U (2021) Lysosome-targeting pH indicator based on peri-fused naphthalene monoimide with superior stability for long term live cell imaging. J Mat Chem B 9(1), 112–124.

Wilden JJ, Jacob JC, Ehrhardt C, Ludwig S, Boergeling Y (2021) Altered signal transduction in the immune response to influenza virus and S. pneumoniae or S. aureus co-Infections. Int J Mol Sci 22(11), 5486.

Zhu W, Cai EL, Li HZ, Wang P, Shen AG, Popp J, Hu JM (2021) Precise encoding of triple-bond Raman scattering of single polymer nanoparticles for multiplexed imaging application. Angew Chem Int Ed 60(40), 21846-21852.

2020

Ali N, Kirchhoff J, Onoja PI, Tannert A, Neugebauer U, Popp J, Bocklitz T (2020) Predictive modeling of antibiotic susceptibility in E. coli strains using the U-Net network and one-class classification. IEEE Access 8, 167711.

Arend N, Pittner A, Ramoji A, Mondol AS, Dahms M, Rüger J, Kurzai O, Schie IW, Bauer M, Popp J, Neugebauer U (2020) Detection and differentiation of bacterial and fungal infection of neutrophils from peripheral blood using Raman spectroscopy. Anal Chem 92(15), 10560−10568.

Baum C, Menezes RC, Svatoš A, Schubert T (2020) Cobamide remodeling in the freshwater microalga Chlamydomonas reinhardtii. FEMS Microbiol Lett 367(20), fnaa171.

Deinhardt-Emmer S, Rennert K, Schicke E, Cseresnyés Z, Windolph M, Nietzsche S, Heller R, Siwczak F, Haupt KF, Carlstedt S, Schacke M, Figge MT, Ehrhardt C, Löffler B, Mosig AS (2020) Co-infection with Staphylococcus aureus after primary influenza virus infection leads to damage of the endothelium in a human alveolus-on-a-chip model. Biofabrication 12(2), 025012.

Diederich B, Lachmann R, Carlstedt S, Marsikova B, Wang H, Uwurukundu X, Mosig AS, Heintzmann R (2020) A versatile and customizable low-cost 3D-printed open standard for microscopic imaging. Nat commun 11(1), 5979.

Dorosz A, Grosicki M, Dybas J, Matuszyk E, Rodewald M, Meyer T, Popp J, Malek K, Baranska M (2020) Eosinophils and neutrophils—molecular differences revealed by spontaneous Raman, CARS and fluorescence microscopy. Cells 9, 2041.

Götz T, Dahms M, Kirchhoff J, Beleites C, Glaser U, Bohnert JA, Pletz MW, Popp J, Schlattmann P, Neugebauer U (2020) Automated and rapid identification of multidrugresistant Escherichia coli against the lead drugs ofacylureidopenicillins, cephalosporins, andfluoroquinolones using specific Raman marker bands. J Biophotonics 13(8), e202000149.

Hengoju S, Wohlfeil S, Munser AS, Shvydkiv O, Boehme S, Beckert E, Tovar M, Roth M, Rosenbaum M (2020) Optofluidic detection setup for multi-parametric analysis of microbiological samples in droplets. Biomicrofluidics 14(2), 024109.

Houhou R, Barman P, Schmitt M, Meyer T, Popp J, Bocklitz T (2020) Deep learning as phase retrieval tool for CARS spectra. Opt Express 28(14), 21002-21024.

Jiang S, Zhao J, Förster R, Weidlich S, Plidschun M, Kobelke J, Andoa RF, Schmidt MA (2020) Three dimensional spatiotemporal nano-scale position retrieval of the confined diffusion of nano-objects inside optofluidic microstructured fibers. Nanoscale 12(5), 3146-3156.

Kirtzel J, Ueberschaar N, Deckert-Gaudig T, Krause K, Deckert V, Gadd GM, Kothe E (2020) Organic acids, siderophores, enzymes and mechanical pressure for black slate bioweathering with the basidiomycete Schizophyllum commune. Environ Microbiol 22(4), 1535-1546.

Koeberle SC, Gollowitzer A, Laoukili J, Kranenburg O, Werz O, Koeberle A, Kipp AP (2020) Distinct and overlapping functions of glutathione peroxidases 1 and 2 in limiting NF-κB-driven inflammation through redox-active mechanisms. Redox Biol 28, 101388.

König A, Hube B, Kasper L (2020) The dual function of the fungal toxin Candidalysin during Candida albicans-macrophage interaction and virulence. Toxins (Basel) 12(8), 469.

Kumamoto CA, Gresnigt MS, Hube B (2020) The gut, the bad and the harmless: Candida albicans as a commensal and opportunistic pathogen in the intestine. Curr Opin Microbiol 56, 7-15.

Overholt WA, Hölzer M, Geesink P, Diezel C, Marz M, Küsel K (2020) Inclusion of Oxford Nanopore long reads improves all microbial and viral metagenome-assembled genomes from a complex aquifer system. Environ Microbiol 22(9), 4000-4013.

Romp E, Arakandy V, Fischer J, Wolz C, Siegmund A, Löffler B, Tuchscherr L, Werz O, Garscha U (2020) Exotoxins from Staphylococcus aureus activate 5-lipoxygenase and induce leukotriene biosynthesis. Cell Mol Life Sci 77(19), 3841–3858.

Schicke E, Cseresnyes Z, Rennert K, Vau V, Haupt KF, Hornung F, Nietzsche S, Swiczak F, Schmidtke M, Koch M, Glück B, Schacke M, Heller R, Mosig AS, Figge MT, Ehrhardt C, Löffler B, Deinhardt-Emmer S (2020) Staphylococcus aureus lung infection results in down-regulation of surfactant protein-A mainly caused by pro-inflammatory macrophages. Microorganisms 8(4), 577.

Schmidt F, Thywißen A, Goldmann M, Cunha C, Cseresnyés Z, Schmidt H, Rafiq M, Galiani S, Gräler MH, Chamilos G, Lacerda JF, Campos Jr A, Eggeling C, Figge MT, Heinekamp T, Filler SG, Carvalho A, Brakhage AA (2020) Flotillin-dependent lipid-raft microdomains are required for functional phagolysosomes against fungal infections. Cell Rep 32(7), 108017.

Shopova IA, Belyaev I, Dasari P, Jahreis S, Stroe MC, Cseresnyés Z, Zimmermann AK, Medyukhina A, Svensson CM, Krüger T, Szeifert V, Nietzsche S, Conrad T, Blango MG, Kniemeyer O, von Lilienfeld-Toal M, Zipfel PF, Ligeti E, Figge MT, Brakhage AA (2020) Human neutrophils produce antifungal extracellular vesicles against Aspergillus fumigatus. mBio 11(2), e00596-20.

Sreekantapuram S*, Lehnert T*, Prauße MTE, Berndt A, Berens C, Figge MT**, Jacobsen ID**; * authors contributed equally; ** corresponding authors (2020) Dynamic interplay of host and pathogens in an avian whole blood model. Front Immunol 11, 500.

Westman J, Walpole GFW, Kasper L, Xue BY, Elshafee O, Hube B, Grinstein S (2020) Lysosome fusion maintains phagosome integrity during fungal infection. Cell Host Microbe 28(6), 798-812.

2019

Bocklitz T (2019) Understanding of non-linear parametric regression and classification models: A taylor series based approach. Proceedings of the 8th International Conference on Pattern Recognition Applications and Methods (ICPRAM 2019) 874-880.

Diederich B, Then P, Jügler A, Förster R, Heintzmann R (2019) cellSTORM-cost-effective super-resolution on a cellphone using dSTORM. PloS one 14(1), e0209827.

Fischer D, Gessnerb G, Fill TP, Barnett R, Tron K, Dornblut K, Kloss F, Stallforth P, Hube B, Heinemann SH, Hertweck C, Scherlach K, Brunke S (2019) Disruption of Membrane Integrity by the Bacterium-Derived Antifungal Jagaricin. Antimicrob Agents Chemother 63(9), e00707-19.

Geesink P, Wegner CE, Probst AJ, Herrmann M, Dam HT, Kaster AK, Küsel K (2019) Genome-inferred spatio-temporal resolution of an uncultivated Roizmanbacterium reveals its ecological preferences in groundwater. Environ Microbiol 22(2), 726-737.

Jügler A, Becker J, Förster R, Heintzmann R (2019) Adaptive holographic region of interest illumination with oblique angles for use in single molecule localization microscopy. SPIE Proc (Optical Society of America, 2019) 11076_1.

Keller S, Wetterhorn KM, Vecellio A, Seeger M, Rayment I, Schubert T (2019) Structural and functional analysis of an l-serine O-phosphate decarboxylase involved in norcobamide biosynthesis. FEBS Lett 593(21), 3040-3053.

Munser AS, Thoß A (2019) Winkelaufgelöste Streulichtanalyse für die Zellbiologie. Photonik 5.

Njeru SN, Kraus J, Meena JK, Lechel A, Katz SF, Kumar M, Knippschild U, Azoitei A, Wezel F, Bolenz C, Leithäuser F, Gollowitzer A, Omrani O, Hoischen C, Koeberle A, Kestler HA, Günes C, Rudolph KL (2019) Aneuploidy-inducing gene knockdowns overlap with cancer mutations and identify Orp3 as a B-cell lymphoma suppressor. Oncogene 39(7), 1445–1465.

Pekmezovic M, Mogavero S, Naglik JR, Hube B (2019) Host–pathogen interactions during female genital tract infections. Trends Microbiol 27(12), 982-996.

Plidschun M, Weidlich S, Šiler M, Weber K, Izmar TC, Schmidt MA (2019) Nanobore fiber focus trap with enhanced tuning capabilities. Opt Express 27(25), 36221.

Ramoji A, Ryabchykov O, Galler K, Tannert A, Markwart R, Requardt RP, Rubio I, Bauer M, Bocklitz T, Popp J, Neugebauer U (2019) Raman spectroscopy follows time-dependent changes in T lymphocytes isolated from spleen of endotoxemic mice. Immunohorizons 3(2), 45-60.

Reupert A, Heck M, Nolte S, Wondraczek L (2019) Side-emission properties of femtosecond laser induced scattering centers in optical fibers. Opt Mater Express 9(6), 2497-2510.

Schubert T, Von Reuß SH, Kunze C, Paetz C, Kruse S, Brand-Schön P, Mac Nelly A, Nüske J, Diekert G (2019) Guided cobamide biosynthesis for heterologous production of reductive dehalogenases. Microb Biotechnol 12(2), 346-359.

Siscar-Lewin S, Hube B, Brunke S (2019) Antivirulence and avirulence genes in human pathogenic fungi. Virulence 10(1), 935-947.

Svensson CM*, Shydkiv O*, Dietrich S, Mahler L, Weber T, Choudhary M, Tovar M, Figge MT**, Roth M**; *authors contributed equally; **corresponding authors (2019) Coding of experimental conditions in microfluidic droplet assays using colored beads and machine learning supported image analysis. Small 15(4), e1802384.

Tannert A, Grohs R, Popp J, Neugebauer U (2019) Phenotypic antibiotic susceptibility testing of pathogenic bacteria using photonic readout methods: recent achievements and impact. Appl Microbiol Biotechnol 103(2), 549-566.

Töpfer N, Müller MM, Dahms M, Ramoji A, Popp J, Slevogt H, Neugebauer U (2019) Raman spectroscopy reveals LPS-induced changes of biomolecular composition in monocytic THP-1 cells in a label-free manner. Integr Biol 11(3), 87–98.

Tovar M, Hengoju S, Weber T, Mahler L, Choudhary M, Becker T, Roth M (2019) One sensor for multiple colors: fluorescence analysis of micro-droplets in microbiological screenings by frequency-division multiplexing. Anal Chem 91(4), 3055-3061.

Tuchscherr L, Pöllath C, Siegmund A, Deinhardt-Emmer S, Hörr V, Svensson CM, Figge MT, Monecke S, Löffler B (2019) Clinical S. aureus isolates vary in their virulence to promote adaptation to the host. Toxins 11(3), 135.

Westman J, Hube B, Fairn GD (2019) Integrity under stress: Host membrane remodelling and damage by fungal pathogens. Cell Microbiol 21(4), e13016.

Wohlfeil S, Hengoju S, Munser AS, Tovar M, Shvydkiv O, Roth M, Schröder S, Beckert E, Eberhardt R, Tünnermann A (2019) Optical fiber based light scattering detection in microfluidic droplets. SPIE Conf Proc 10875.

Wondraczek L, Pohnert G, Schacher FH, Köhler A, Gottschaldt M, Schubert US, Küsel K, Brakhage AA (2019) Artificial microbial arenas: materials for observing and manipulating microbial consortia. Adv Mater 31(24), e1900284.

Wondraczek L, Gründler A, Reupert A, Wondraczek K, Schmidt MA, Pohnert GA, Nolte S (2019) Biomimetic light dilution using side-emitting optical fber for enhancing the productivity of microalgae reactors. Sci Rep 9(1), 9600.

2018

Ali N, Girnus S, Rösch P, Popp J, Bocklitz T (2018) Sample-size planning for multivariate data: A Raman-spectroscopy-based example. Anal Chem 90(21), 12485–12492.

Bocklitz T, Meyer T, Schmitt M, Rimke I, Hoffmann F, Von Eggeling F, Ernst G, Guntinas-Lichius O, Popp J (2018) Invited Article: Comparison of hyperspectral coherent Raman scattering microscopies for biomedical applications. APL Photonics 3, 092404.

Geesink P, Tyc O, Küsel K, Taubert M, Van de Velde C, Kumar S, Garbeva P (2018) Growth promotion and inhibition induced by interactions of groundwater bacteria. FEMS Microbiol Ecol 94(11).

Guo S, Pfeifenbring S, Meyer T, Ernst G, Von Eggeling F, Maio V, Massi D, Cicchi R, Pavone FS, Popp J, Bocklitz T (2018) Multimodal image analysis in tissue diagnostics for skin melanoma. J Chemometr 32, e2963.

Jung EM, Kothe E, Raudaskoski M (2018) The making of a mushroom: Mitosis, nuclear migration and the actin network. Fungal Genet Biol 111, 85-91.

Kasper L, König A, Koenig, Gresnigt M, Westman, Drummond, Lionakis, Groß, Ruland, Naglik, Hube B (2018) The fungal peptide toxin Candidalysin activates the NLRP3 inflammasome and causes cytolysis in mononuclear phagocytes. Nat Commun 9(1), 4260.

Majzner K, Tott S, Roussille L, Deckert V, Chlopicki S, Baranska M (2018) Uptake of fatty acids by a single endothelial cell investigated by Raman spectroscopy supported by AFM. Analyst 143(4), 970–980.

Meyer R, Yao X, Deckert V (2018) Latest instrumental developments and bioanalytical applications in tip-enhanced Raman spectroscopy. Trend Anal Chem 102, 250–258.

Richardson, Mogavero S, Moyes, Blagojevic, Krüger, Verma, Coleman, De La Cruz Diaz, Schulz, Ponde, Carrano, Kniemeyer O, Wilson, Bader, Enoiu, Ho, Kichik, Gaffen, Hube B, Naglik JR (2018) Processing of Candida albicans Ece1p Is critical for candidalysin maturation and fungal virulence. mBio 9(1), e02178-17.

Rodner E, Bocklitz T, Von Eggeling F, Ernst G, Chernavskaia O, Popp J, Denzler J, Guntinas-Lichius O (2018) Fully convolutional networks in multimodal nonlinear microscopy images for automated detection of head and neck carcinoma: Pilot study. Head Neck 41(1), 116-121.

Schmidt H, Vlaic S, Krüger T, Schmidt F, Balkenhol J, Dandekar T, Guthke R, Kniemeyer O, Heinekamp T, Brakhage AA (2018) Proteomics of Aspergillus fumigatus conidia-containing phagolysosomes identifies processes governing immune evasion. Mol Cell Proteomics 17(6), 1084-1096.

Schneidewind H, Zeisberger M, Plidschun M, Weidlich S, Schmidt MA (2018) Photonic candle – focusing light using nano-bore optical fibers. Opt Express 26(24), 31706-31716.

Svensson CM, Medyukhina A, Belyaev I, Al-Zaben N, Figge MT (2018) Untangling cell tracks: Quantifying cell migration by time lapse image data analysis. Cytometry A 93(3), 357-370.

Tannert A, Ramoji A, Neugebauer U, Popp J (2018) Photonic monitoring of treatment during infection and sepsis: development of new detection strategies and potential clinical applications. Anal Bioanal Chem 410(3), 773-790.

Tovar M, Weber T, Hengoju S, Lovera A, Munser AS, Shvydkiv O, Roth M (2018) 3D-glass molds for facile production of complex droplet microfluidic chips. Biomicrofluidics 12(2), 024115.

Voltersen V, Blango MG, Herrmann S, Schmidt F, Heinekamp T, Strassburger M, Krüger T, Bacher P, Lother J, Weiss E, Hünniger K, Liu H, Hortschansky P, Scheffold A, Löffler J, Krappmann S, Nietzsche S, Kurzai O, Einsele H, Kniemeyer O, Filler SG, Reichard U, Brakhage AA (2018) Proteome analysis reveals the conidial surface protein CcpA essential for virulence of the pathogenic fungus Aspergillus fumigatus. mBio 9(5), e01557.

2017

Ashtikar M, Langelüddecke L, Fahr A, Deckert V (2017) Tip-enhanced Raman scattering for tracking of invasomes in the stratum corneum. Biochim Biophys Acta Gen Subj 1861(11), 2630-2639.

Bocklitz T, Schmitt M, Lukić A, Heuke S, Chernavskaia O, Meyer T, Popp J (2017) Non-linear multimodal imaging for disease diagnostics and treatment monitoring. International Conference on Biophotonics V SPIE Proc 10340.

Chernavskaia O, Guo S, Meyer T, Vogler N, Akimov D, Heuke S, Heintzmann R, Bocklitz T, Popp J (2017) Correction of mosaicking artifacts in multimodal images caused by uneven illumination. J Chemometr 31(6), e2901.

Deckert-Gaudig T, Taguchi A, Kawata S, Deckert V (2017) Tip-enhanced Raman spectroscopy – from early developments to recent advances. Chem Soc Rev 46(13), 4077-4110.

Naglik JR, König A, Hube B, Gaffen SL (2017) Candida albicans–epithelial interactions and induction of mucosal innate immunity. Curr Opin Microbiol 40, 104-112.

Richard-Lacroix M, Zhang Y, Dong Z, Deckert V (2017) Mastering high resolution tip-enhanced Raman spectroscopy: towards a shift of perception. Chem Soc Rev 46(13), 3922-3944.

2016

Moyes DL, Wilson D, Richardson JP, Mogavero S, Tang SX, Wernecke J, Höfs S, Gratacap RL, Robbins J, Runglall M, Murciano C, Blagojevic M, Thavaraj S, Förster TM, Hebecker B, Kasper L, Vizcay G, Iancu SI, Kichik N, Häder A, Kurzai O, Luo T, Krüger T, Kniemeyer O, Cota E, Bader O, Wheeler RT, Gutsmann T, Hube B, Naglik JR (2016) Candidalysin is a fungal peptide toxin critical for mucosal infection. Nature 532(7597), 64-68.