iTag - Elucidating infection processes by super-resolution coherent Raman imaging of alkyne-tagged biomarkers

The aim of the iTag project is to detect a large variety of small signal molecules used for communication within microbiological communities or between microorganisms and higher organisms. The sensitivity of high-resolution coherent Raman scattering (CRS) will be increased with novel laser sources to detect low molecular weight substances in low concentrations. In addition, the sensitivity of CRS will be brought below Abbe's limit by small tailor-made modifications of the target molecules that do not alter their functionality or affect the cells, but deliver strong Raman signals. These so-called Raman tags allow the simultaneous investigation of many different molecules. These tags are used to detect virulence factors and antimicrobial substances non-invasively in vivo with minimal influence on the biological system under investigation. The project will provide new insights into the spatial distribution and dynamics of small molecule biomarkers in microbial cells and consortia, e.g. in infection processes, which are difficult to obtain by other means.

Participating Scientists

Prof. Dr. Jürgen Popp

Prof. Dr. Jürgen Popp

Project Coordinator

Leibniz-IPHT • Spectroscopy / Imaging

juergen.popp@leibniz-ipht.de

Prof. Dr. Christian Hertweck

Prof. Dr. Christian Hertweck

Principal Investigator

Leibniz-HKI • Biomolecular Chemistry

christian.hertweck@leibniz-hki.de

Tobias Meyer

Dr. Tobias Meyer

Principal Investigator

Leibniz-IPHT • Spectroscopy / Imaging

tobias.meyer@leibniz-ipht.de